A new breakthrough strategy strengthens CRISPR editing, minimizing large deletions and enhancing safety and accuracy in genetic modifications. Credit: 2024 KAUST
This approach tackles a critical issue with CRISPR technology: the act of slicing the genome at specific points and then rejoining it, which inherently risks damaging theHoping to mitigate this issue, a team led by Mo Li, a stem cell biologist at KAUST, investigated DNA repair pathways that lead to large genomic deletions following CRISPR editing in human stem cells.
“This easy-to-use approach could reduce the chances of these harmful large DNA deletions from happening,” says Baolei Yuan, a former Ph.D. student in Li’s lab and one of the architects of the study, along with Chongwei Bi and Yeteng Tian from Li’s lab.Moreover, these same interventions were found to enhance the efficiency of homology-directed repair, a mechanism known for its ability to enable accurate genome editing without adding unintended mutations.
With a provisional patent application filed for this innovative strategy, the team continues to explore the mechanisms behind a wider array of undesirable mutations and to hone its techniques for making CRISPR safer and more efficient.
Technology Technology Latest News, Technology Technology Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Source: ScienceDaily - 🏆 452. / 53 Read more »