Optics and AI find viruses faster | ScienceDaily

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Researchers have developed an automated version of the viral plaque assay, the gold-standard method for detecting and quantifying viruses.

The new method uses time-lapse holographic imaging and deep learning to greatly reduce detection time and eliminate staining and manual counting. This advance could help streamline the development of new vaccines and antiviral drugs.

"By cutting down the detection time compared to traditional viral plaque assays, this technique might help expedite vaccine and drug development research by significantly reducing the detection time needed and eliminating chemical staining and manual counting entirely, explains Li.

The new stain-free automated viral plaque assay system replaces manual plaque counting with a lens-free holographic imaging system that images the spatiotemporal features of PFUs during incubation. A deep learning algorithm is then used to detect, classify and locate PFUs based on changes observed.esicular stomatitis

 

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