Researchers develop versatile and low-cost technology for targeted long-read RNA sequencing: The easy-to-implement technology reduces the per-reaction cost of target capture by 2-3 orders of magnitude, as compared to standard commercial solutions -- ScienceDaily

In a development that could accelerate the discovery of new diagnostics and treatments, researchers at Children's Hospital of Philadelphia have developed a versatile and low-cost technology for targeted sequencing of full-length RNA molecules. The technology, called TEQUILA-seq, is highly cost-effective compared to commercially available solutions for targeted RNA sequencing and can be adapted for different research and clinical purposes.

"Targeted long-read RNA sequencing is a powerful strategy for elucidating the RNA repertoire for any predefined set of genes. However, existing technologies for targeted sequencing of full-length RNA molecules are either expensive or difficult to set up, putting them out of reach for many labs," said co-senior author Lan Lin, PhD, Assistant Professor of Pathology and Laboratory Medicine and a member of the Raymond G. Perelman Center for Cellular and Molecular Therapeutics at CHOP.

To address this limitation, the CHOP researchers developed TEQUILA-seq . A key innovation in TEQUILA-seq is a nicking-endonuclease triggered isothermal strand displacement amplification reaction, which can synthesize large quantities of biotinylated capture probes from a cheap pool of non-biotinylated oligos as the templates. Using an input of only 2 ng of template oligos, the researchers can generate 25 ug of TEQUILA probes, which can be used for at least 250 capture reactions.

To illustrate its biomedical utility, the researchers applied TEQUILA-seq to profile full-length RNA molecules of 468 actionable cancer genes across 40 breast cancer cell lines. They discovered previously unknown transcript isoforms in extensively studied cancer genes that may shed light on how genes that protect the body from cancer are inactivated in individual tumors.

Technology Technology Latest News, Technology Technology Headlines

Similar News:You can also read news stories similar to this one that we have collected from other news sources.

Carbon-based quantum technology: Researchers contact single graphene nanoribbons -- ScienceDailyGraphene nanoribbons have outstanding properties that can be precisely controlled. Researchers have succeeded in attaching electrodes to individual atomically precise nanoribbons, paving the way for precise characterization of the fascinating ribbons and their possible use in quantum technology.
Source: ScienceDaily - 🏆 452. / 53 Read more »

Researchers develop versatile and low-cost technology for targeted long-read RNA sequencingIn a development that could accelerate the discovery of new diagnostics and treatments, researchers at Children's Hospital of Philadelphia (CHOP) have developed a versatile and low-cost technology for targeted sequencing of full-length RNA molecules. The technology, called TEQUILA-seq, is highly cost-effective compared to commercially available solutions for targeted RNA sequencing and can be adapted for different research and clinical purposes. The details are described in a paper in Nature Communications.
Source: physorg_com - 🏆 388. / 55 Read more »