TrEMOLO: accurate transposable element allele frequency estimation using long-read sequencing data combining assembly and mapping-based approaches - Genome Biology

] and the following options: -t 19000000000, -t 15000000000, -t 12000000000, -t 10000000000, -t 5000000000, -t 2000000000). This subsampling resulted in a series of sequencing datasets with more or less degraded depths ranging from 183x to 15x.Genomic DNA was extracted from 50 mg of G0-F100 and G73 male flies using the Nanobind Tissue Big DNA Kit with Insect buffer as lysis buffer.

For droplet generation, 20 μl of each mixture was transferred to the center line of a DG8 droplet generator cartridge . Then, 70 μl of droplet generation oil for the probe was injected in the cartridge bottom line and droplets were produced using a QX200 Droplet Generator .

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